Evaluation of a Beta-propeller Phytase for its Application in Aquaculture

Autores/as

  • Martha Guerrero-Olazarán Universidad Autónoma de Nuevo León
  • L. Elizabeth Cruz-Suárez Universidad Autónoma de Nuevo León
  • Denis Ricque-Marie Universidad Autónoma de Nuevo León
  • Martha Nieto-López Universidad Autónoma de Nuevo León
  • Mireya Tapia-Salazar Universidad Autónoma de Nuevo León
  • Eddy L. Cab-Barrera Universidad Autónoma de Nuevo León
  • José M. Viader-Salvadó Universidad Autónoma de Nuevo León

Palabras clave:

Phytase, Soybean meal, Pea protein

Resumen

The beta-propeller phytases are structurally different from the commercially available phytases, possess high thermal stability, an optimal temperature of 55 to 70°C, unique Ca2+-dependent catalytic properties, a pH optimum close to 7, and exhibit activity within a range of pHs that is broader than those of the histidine acid phytases. In this work, the performance of FTEII, a new beta-propeller phytase, was compared with those of three commercial phytases in terms of thermostability at 99°C, resistance to proteolysis by digestive enzymes, and their effectiveness for phosphorus (P) release from two feed ingredients.
FTEII showed the highest thermostability with residual activity of 82±3 after 1.5 min treatment at 90°C. All phytases were resistant to shrimp digestive enzymes and to porcine trypsin, residual activities showed values higher than 60% in all cases. Total phosphorus released from each phytase-treated ingredient was time, temperature and type of ingredient dependent. FTEII treatments showed the higher levels of phosphorus release in both, soybean meal (68%) and pea (57%) at 50°C. Endogenous hydrolytic activity of both ingredients promoted a phosphorus release, up to 28% or 17% of total phosphorus in soybean meal or in pea protein concentrate, respectively. Unlike commercial phytases, FTEII is phytate specific; therefore it is possible to assume that most of the phosphorus released came only from phytate. FTEII offer an alternative as phytase additive for pelleted feeds and for releasing phosphorus under conditions suitable for digestive tracts of species grown in aquaculture.

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Citas

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Publicado

2013-11-30

Cómo citar

Guerrero-Olazarán, M., Cruz-Suárez, L. E., Ricque-Marie, D., Nieto-López, M., Tapia-Salazar, M., Cab-Barrera, E. L., & Viader-Salvadó, J. M. (2013). Evaluation of a Beta-propeller Phytase for its Application in Aquaculture. Avances En Nutrición Acuicola. Recuperado a partir de https://nutricionacuicola.uanl.mx/index.php/acu/article/view/79

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