Development of a Low-fish Meal Feed Formulation for Commercial Production of Litopenaeus vannamei
Palabras clave:shrimp fish meal replacement
Fish meal is used in marine shrimp feeds because it is high in protein, highly digestible and is an effective
feed attractant. Reasons for current interest in its replacement include irregular availability, variable quality,
perceived contribution to deterioration of fisheries, potential for adulteration, contamination with
hydrocarbons and biological pathogens, and increasing cost. Growth trials were conducted with juvenile
Litopenaeus vannamei to evaluate modifications in nutrient supplementation/restriction of a basal fish meal
replacement formula at the 50% replacement level. Trials compared growth and survival of shrimp fed a
basal control feed to experimental feeds in which crystalline amino acids (CAA), oil and lecithin, blood meal
and specific minerals were modified. Improved or similar growth relative to the control feed were shown
with reduced levels of CAA, removal of menhaden oil, inclusion of a lower level of lecithin, and maintenance
of blood meal and mineral supplements at their basal levels. This information was used to develop a
generalized fish meal replacement formulation for marine penaeid shrimp using readily available partiallypurified
ingredients. Low- and high-marine animal meal feed formulations are compared.
Akiyama D.M., Coelho, S.R., Lawrence, A.L. and E.H. Robinson.(1989) Apparent digestibility of feedstuffs
by the marine shrimp Penaeus vannamei Boone. Nippon Suisan Gakkaishi 55: 91-98.
Barlow, S.M. (2000) Fish and oil. Global Aquaculture Advocate 3(2);85-88.
Booth, M.A., Allan, G.L. (2001) Replacement of fish meal in diets for Australian silver perch, Bidyanus
bidyanus. Aquaculture 196, 67-85.
Borlongan, I.G., Eusebio, P.S., Welsh, T. (2003) Poetential of feed pea (Pisum sativum) meal a a protein
source in practical diets for milkfish (Chanos chanos Forsskal). Aquaculture 225, 89-98.
Brunson, J.F., Romaire, R.P., Reigh, R.C. (1997) Apparent digestibility of selected
ingredients in diets for white shrimp Penaeus setiferus L. Aquaculture Nutrition 3, 9-16.
Canadian Food Inspection Agency (CFIA) (1999) Summary report of contaminant results in fish feed,
fishmeal and fish oil. http://www.inspection.gc.ca/english/anima/feebet/dioxe.shtml.
Chen, H.Y., Leu, Y.T., Roelants, I. (1992) Quantification of arginine requirement of juvenile
marine shrimp Penaeus monodon using microencapsulated arginine. Marine
Biology 114: 229-233.
Chou, R.L, Her, B.Y., Su, M.S., Hwang, G., Wu, Y.H., Chen, H.Y. (2004) Substituting fish meal with
soybean meal in diets of juvenile cobia Rachycentron candum. Aquaculture 229, 325-333.
Coman, C.J., Sarac, H.Z., Fielder, D., Thorne, M. (1996) Evaluation of crystalline amino acids, betaine, and
AMP as food attractants of the giant tiger prawn (Penaeus monodon). Comparative Biochemical
Physiology 113A, 247-253.
Cowey, C.B.(1990) The present status and problems of world aquaculture with special
reference to fish feeds. Aquaculture in the UK. In: The Current Status of Fish
Nutrition in Aquaculture (ed. by M. Takeda and T. Watanabe)m oo, 13-26, Japan Translation Center, Tokyo.
Cruz-Suarez, L.E., Ricque-Marie, D., Tapia-Salzar, M., McCallum, I.M., Hickling, D., (2001) Assessment of
differenctly processed feed pea (Pisum sativum) meals and canola meal (Brassica sp.) in the diets for
blue shrimp (Litopenaeus stylirostris). Aquaculture 196, 87-104.
Davis, D.A., Arnold, C.R. (2000) Replacement of fish meal in practical diets for the Pacific white shrimp,
Litopenaeus vannamei. Aquaculture 185, 291-298.
Day, R.W., Quinn, G.P. (1989) Comparison of treatments after and analysis of variance in ecology.
Ecological Monographs 59, 433-463.
Delgado, C., Rosegrant, M., Wada, N., Meijer, S. , Ahmed, M. (2002) Fish as food: projections to 2020 under
different scenarios. Markets and Structural Division. International Food Policy Research Institute.
Easton, M.D., Luszniak, D., Von der,. G.E. (2002) Preliminary examination of contaminant loadings in
farmed salmon, wild salmon and commercial salmon feed. Chemosphere 46(7), 1053-1074.
Eusebio, P., Coloso, R.M. (1998) Evaluation of leguminous seed meals and leaf meals as plant protein
sources in diets for juvenile Penaeus indicus. Israeli Journal of Aquaculture-Bamidgeh 40, 47-54.
FAO (2002) World review of fisheries and aquaculture. Part 1. Fisheries resources: trends in production,
utilization and trade. http://www.fao.org/docrep/005/y7300e/y7300e00.htm
Forster, I., Dominy, W. (2001) Rendered non-marine animal by-products as shrimp feed components – Final
Report. Poultry Protein and Fat Council, U.S. Poultry and Egg Association. Tucker, Georgia, USA.
Forster, I., Dominy, W., Obaldo, L., Tacon, A.G.J. (2002) Rendered meat and bone meals as ingredients of
diets for shrimp Litopenaeus vannamei (Boone, 1931). Aquaculture 219, 655-670.
Fox, J.M., Lawrence, A.L., Li-Chan, E. (1995) Dietary requirement for lysine by juvenile Penaeus vannamei
using intact and free amino acid sources. Aquaculture 131: 279-290.
Glencross, B., Evans, D., Hawkins, W., Jones, B. (2003) Evaluation of dietary inclusion of yellow lupin
(Lupinus luteus) kernel meal on the growth, fed utilization and tissue histolory of rainbow trout
(Oncorhynchus mykiss). Aquaculture 235, 411-422.
Gong, H., Lawrence, A.L., Jian, D.H., Castille, F.L., and Gatlin, D.M. 2000. Lipid nutrition of juvenile
Litopenaeus vannamei. I. Dietary cholesterol and de-oiled soy lecithin requirements and their
interaction. Aquaculture 190, 305-324.
Guillaume, J., Kaushik, S., Bergot, P., Metailler, R. (2001) Nutrition and feeding of fish and crustaceans.
Springer-Verlag, Chichester, UK.
Hardy, R.W. (2001) Urban legends and fish nutrition, Part 2. Aquaculture Magazine 27, 57-60.
He, H., Lawrence, , A. L., Liu, R.. (1992). Evaluation of dietary essentiality of fat-soluble vitamins, A, D, E
and K for penaeid shrimp (Penaeus vannamei). Aquaculture 103: 177-185.
Hertrampf, J.W., Piedad-Pascual, F. (2000) Handbook on Ingredients for Aquaculture Feeds. Kulwer
Academic Publishers. Dordrecht, The Netherlands.
Houser, R.H., Akiyama, D.M. (1997) Feed formulation principles. In: Crustacean Nutrition (eds. L.
D’Abramo, D.E. Conklin, D.M. Akiyama), pp. 493-519. The World Aquaculture Society, Baton
Rouge, Louisiana, USA.
Jacobs, M.N., Covaci, A., Schepens, P. (2002) Investigation of selected persistent organic pollutants in
farmed Atlantic salmon (Salmo salar), salmon aquaculture feed, and fish oil components of the feed.
Environ Sci Technol. 36(13), 797-805.
Kaushik, S.J., Coves, D., Dutto, G., Blanc, D. (2004) Almost total replacement of fish meal by plant protein
sources in the diet of the marine teleost, the European seabass, Dicentrarchus labrax. Aquaculture
Lim, C. (1996) Substitution of cottonseed meal for marine animal protein in diets for Penaeus vannamei. J.
World Aquacult. Soc. 27, 402-409.
Lim, C., Dominy, W. (1990) Evaluation of soybean meal as a replacement for marine and animal protein in
diets for shrimp Penaeus vannamei. Aquaculture 87, 53-64.
Meilahn, C.W., Davis, D.A., Arnold, C.R. (1996) Effects of commercial fish meal analogue and menhaden
fish meal on growth of red drum fed isonitrogenous diets. The Progressive Fish Culturist 58, 111-
Millamena, O.M., Bautista-Tauerel, M.N., Kanazawa, A. (1996) Methionine requirement of juvenile tiger
shrimp Penaeus monodon Fabricius. Aquaculture 143, 403-410.
Naylor, R.L., Goldburg, R.J, Primavera, J.H., Kautsky, N., Beveridge, M.C.M., Clay, J., Folke, C.,
Lubchenco, J., Mooney, H., Troell, M., 2000. Effect of aquaculture on world fish supplies. Nature
NRC, 1993. Nutrient requirements of fish. National Academy Press. Washington, D.C. USA.
Penaflorida, V.D. (1995) Growth and survival of juvenile tiger prawn fed diets where fish meal is partially
replaced with papaya (Carica papaya L.) or camote (Ipomea batatas Lam.) leaf meal. Isr. J.
Aquacult. Bamidgeh 47, 25-33.
Piedad-Pascual, F., Cruz, E.M., Sumalangcay, A. (1995) Supplemental feeding of Penaeus monodon juveniles
with diets containing various levels of defated soybean meal. Aquaculture 89, 183-191.
Pike, I.H. (2000). http://www.fishfacts.com/sfdpriv/news1archive2000/
Pike, I.H. (1999). Health benefits from feeding fish oil and fish meal: The role of long chain omega-3
polyunsaturated fatty acids in animal feeding. International Fish Meal and Oil Manufacturers
Association. Herts, UK.
Pike, I. H. (1998) Fishmeal outlook. Int. Aquafeeds 1, 5-8.
Pontecorvo, G. (2001) Supply side uncertainty and the management of commercial fisheries: Peruvian
anchovetta, an illustration. Marine Policy 25, 169-172.
Samocha T.M, Davis, D.A., Saoud, I.P., DeBault, K. DeBault (2004) Substitution of fish meal by co-extruded
soybean poultry by-product meal in practical diets for the Pacific white shrimp, Litopenaeus
vannamei. Aquaculture 231, 197-203.
Clesceri, L.S., Greenberg, A.E., Eaton, A.D. (1998). Standard methods for the examination of water and
wastewater. 20th Edition, American Public Health Association. Washington, D.C. USA.
Sudaryono, A., Hoxey, M.J., Kailis, S.G., Evans, L.H. (1995) Investigation of alternative protein sources in
practical diets for juvenile shrimp Penaeus monodon. Aquaculture 134, 313-323.
Sudaryono, A., Tsvetnenko, E., Hutabarat, J, Supriharyono, A. (1999) Lupin ingredients in shrimp (Penaeus
monodon) diets: influence of lupin species and types of meals. Aquaculture 171, 121-133.
Tacon, A. (1998) FAO aquaculture production update. Int. Aquafeeds 2, 13-16.
Tacon, A. (1999) Estimated global aquafeed production and aquaculture in 1997 and projected growth. Int.
Aquafeed 2, 5.
Tacon, A.G.J. and U.C. Barg (1998) Major challenges to feed development for marine and diadromous finfish
and crustacean species. In: Tropical Mariculture (ed. by S.S. de Silva) pp. 171-208. Academic Press,
Tidwell, J.H., Webster, C.D., Yancey, D.H., D’Abramo, L.R. (1993) Partial and total replacement of fish
meal with soybean meal and distillers’ by-products in diets for pond culture of the freshwater prawn
(Macrobrachium rosenbergii). Aquaculture 118, 119-130.
Williams, K. (2004) Fish meal in aquaculture feeds for barramundi. In: Fisheries Research and Development
Report 93/120-04. http://www.frdc.com.au/pub/reports/files/93-120-04.htm
Yang, Y. Xie, S., Lei, W., Zhu, X., Yan, Y. (2003) Effect of replacement of fish meal by meat and bone meal
and poultry by-product meal in diets on the growth and immune response of Macrobrachium
nipponense. Fish & Shellfish Immunology 17, 105-114.