Cholecystokinin and Trypsin Responses of Larval Red Drum (Sciaenops Ocellatus) to Soluble Components of Rotifers (Brachionus Plicatilis) and Algae (Isochrysis Galbana)
Palabras clave:
cholecystokinin, trypsin, digestive physiologyResumen
In an attempt to better understand the problems in weaning larval fish to artificial diets, our lab has begun to
investigate the role of the digestive hormone cholecystokinin (CCK). While there are a number of other labs also
investigating CCK and other digestive hormones such as bombesin, PPY, and gastrin; research into the roles of these
hormones in fish is still in its infancy. Previous research with red drum larvae suggests that some component of
rotifers and algae enable red drum larvae to more efficiently utilize microparticulate diets than when these are not
included in the culture system. The current study investigated the impact of soluble components of rotifers and algae
on the CCK and trypsin responses of larval red drum at 6 and 10 days post hatch (DPH). Introduction of
homogenized rotifers was shown to significantly increase whole body CCK levels, CCK mRNA, and trypsin activity
in 6 DPH red drum larvae, but not in 10 DPH larvae. Homogenates of Isochrysis galbana did not significantly affect
CCK or trypsin at either age. This research suggests that there is a soluble component of rotifers that can upregulate
digestive function in larval red drum, at least in 6 DPH larvae.
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Cahu, C. and J.Z. Infante, 2001. Substitution of live food by formulated diets in marine fish larvae. Aquaculture
(1-2): p. 161-180.
Cahu, C., et al., 1998. Preliminary results on sea bass (Dicentrarchus labrax) larvae rearing with compound diet
from first feeding. Comparison with carp (Cyprinus carpio) larvae. Aquaculture 169(1-2): p. 1-7.
Holt, G.J., 1993. Feeding larval red drum on microparticulate diets in a closed recirculating water system. J World
Aquaculture Soc. 24(2): p. 225-230.
Lazo, J.P., et al., 2000. Co-feeding microparticulate diets with algae: toward eliminating the need of zooplankton
at first feeding in larval red drum (Sciaenops ocellatus). Aquaculture 188(3-4): p. 339-351.
Lazo, J.P., G.J. Holt, and C.R. Arnold, 2000. Ontogeny of pancreatic enzymes in larval red drum Sciaenops
ocellatus. Aquaculture Nutrition 6(3): p. 183-192.
Cahu, C., et al., 2004. Expression and activities of pancreatic enzymes in developing sea bass larvae
(Dicentrarchus labrax) in relation to intact and hydrolyzed dietary protein; involvement of cholecystokinin.
Aquaculture 238(1-4): p. 295-308.
Govoni, J., G. Boehlert, and Y. Watanabe, 1986. The physiology of digestion in fish larvae. Environ. Biol. Fish.
(1): p. 59-77.
Ribeiro, L., et al., 1999. Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858.
Aquaculture 179(1-4): p. 465-473.
Yufera, M. and M.J. Darias, 2007. The onset of exogenous feeding in marine fish larvae. Aquaculture 268(1-4): p.
-63.
Rajjo, I.M., S.R. Vigna, and J.W. Crim, 1988. Actions of cholecystokinin-related peptides on the gallbladder of
bony fishes in vitro. Comparative Biochemistry and Physiology, C 90C(1): p. 267-273.
Einarsson, S., P.S. Davies, and C. Talbot, 1997. Effect of exogenous cholecystokinin on the discharge of the
gallbladder and the secretion of trypsin and chymotrypsin from the pancreas of the Atlantic salmon, Salmo
salar L. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 117(1): p. 63-67.
Olsson, C. and S. Holmgren, 2001. The control of gut motility. Comp. Biochem. Physiol. Physiol. 128(3): p. 479-
Gelineau, A. and T. Boujard, 2001. Oral administration of cholecystokinin receptor antagonists increase feed
intake in rainbow trout. Journal of Fish Biology 58(3): p. 716-724.
Rojas-Garcia, C.R. and I. Rønnestad, 2002. Cholecystokinin and tryptic activity in the gut and body of
developing Atlantic halibut larvae: evidence for participation in the regulation of protein digestion. J. Fish
Biol. 61(4): p. 973-986.
Koven, W., et al., 2002. Stimulatory effect of ingested protein and/or free amino acids on the secretion of the
gastro-endocrine hormone cholecystokinin and on tryptic activity, in early-feeding herring larvae, Clupea
harengus. Marine Biology 140(6): p. 1241-1247.
Webb, K.A., 2008. Cholecystokinin and the Ontogeny of Digestion in the Red Drum (Sciaenops ocellatus).
University of Texas at Austin, Port Aransas, TX, USA, 168.
Liddle, R.A., 1997. Cholecystokinin Cells. Annual Review of Physiology 59(1): p. 221-242.
Liddle, R.A., 2000. Regulation of cholecystokinin secretion in humans. Journal of Gastroenterology 35(3): p.
-187.
Lazo, J.P., 1999. Development of the digestive system in red drum (Sciaenops ocellatus) larvae. Ph.D.
Dissertation, The University of Texas at Austin, Austin, 212.
Lazo, J.P., G.J. Holt, and C.R. Arnold, 2002. Towards the development of suitable microdiets for substitution of
live prey in the rearing of red drum (Sciaenops ocellatus) larvae: Applications of studies on digestive
physiology. Int. Commemorative Symp. -- 70th Anniversary of the Japan Society of Fisheries Science,
Yokohama (Japan), 1-5 Oct 2002: p. Vol. 68, suppl. 1, p.
Srivastava, A., et al., 2006. Protein content and amino acid composition of the live feed rotifer (Brachionus
plicatilis): With emphasis on the water soluble fraction. Aquaculture 254(1-4): p. 534-543.
Hamana, K. and M. Niitsu, 2006. Cellular polyamines of lower eukaryotes belonging to the phyla Glaucophyta,
Rhodophyta, Cryptophyta, Haptophyta
