Un Estudio de Caso en el Manejo del Virus del Síndrome de la Mancha Blanca en una Granja de Centroamérica
Keywords:
virus del síndrome de la mancha blanca, Litopenaeus vannameiAbstract
En una granja de cultivo intensivo de camarón en Panamá, Centroamérica, donde la supervivencia de
Litopenaeus vannamei había sido reducida a menos de 10% a lo largo de varios ciclos de cultivo a causa del
virus del síndrome de la mancha blanca (WSSV), principalmente, se utilizaron geomembranas de polietileno
para la construcción de 18 estanques de maternidad de 0.04 ha y 18 estanques de engorda de 0.1 ha, los cuales
fueron sembrados a tasas de 274-371 organismos/m2 y 123-130 organismos/m2, respectivamente. Se
emplearon técnicas para la exclusión de agentes patógenos y sus vectores potenciales en suelo, agua y aire. Se
obtuvo una supervivencia promedio de 80%, utilizando sistemas de cultivo sin intercambio de agua y
postlarvas provenientes de camarones reproductores silvestres, así como postlarvas provenientes de
reproductores domesticados y libres de enfermedades, cuyas producciones promedio fueron de 11,086 kg/ha y
13,241 kg/ha, respectivamente. En estanques de tierra sembrados de forma concurrente en la misma granja, la
supervivencia promedio fue de sólo 8.7%. El manejo de granjas de camarón en áreas infectadas con WSSV es
tratado en el presente trabajo. Los alentadores resultados obtenidos en estos ensayos se discuten en el contexto
de información reciente con respecto a la transmisión de WSSV, la identificación de sus vectores y
reservorios potenciales.
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References
Achuthankutty, C.T., and U.M. Desai. 2004. Treatment of white spot syndrome virus (WSSV) in penaeid
shrimp culture. Proceedings of the National Seminar on New Frontiers in Marine Bioscience
Research, Jan. 22-23, 2004, pp. 63-67.
Boyd, C.E. 2002. Correct liming improves pond water, bottom quality. Global Aquaculture Advocate, Aug.
, pp. 58-59.
Briggs, M. and S. Funge-Smith. 2003. The introduction of Penaeus vannamei and P. stylirostris into the
Asia-Pacific Region. Presented at the International Workshop on International Mechanisms for the
Control and Responsible Use of Alien Species in Aquatic Ecosystems, Xishangbanna, China, August
-29, 2003 (to be published as an FAO Report).
Corbel, V., L. Zuprizal, Z. Shi, C. Huang, L. Sumartono, J.-M. Arcier and J.-R. Bonami. 2001. Experimental
infection of European crustaceans with white spot syndrome virus (WSSV). Journal of Fish Disease
(7): 377-382.
Chakraborty, A., S.K. Otta, B. Joseph, S. Kumar, Md. Shahadat, I. Hossain, I. Karunasagar, M.N. Venugopal
and I. Karunasagar. 2002. Prevalence of white spot syndrome virus in wild crustaceans along the
coast of India. Current Science 82(11): 1392-1397.
Chang, P.-S., H.-C.Chen and Y-C. Wang. 1998. Detection of white spot syndrome associated baculovirus in
experimentally infected wild shrimp, crab and lobsters by in situ hybridization. Special Isue:
Proceedings of the 2nd International Conference on the Culture of Penaeid Prawns and Shrimps, eds.
E.T. Quinitio and J.H. Primavera. Aquaculture 164(1-4): 233-242.
Chang, Y.-S., C.-F. Lo, S-E. Peng, K.-F. Liu, C.-H. Want and G.-H. Kou. 2002. White spot syndrome virus
(WSSV) PCR-positive Artemia cysts yield PCR-negative nauplii that fail to transmit WSSV when
fed to shrimp postlarvae. Diseases of Aquatic Organisms 49(1):1-10.
Chang, C., M. Su, H. Chen and I. Liao. 2003. Dietary beta-1,3glucan effectively improves immunity and
survival of Penaeus monodon challenged with white spot syndrome virus. Fish and Shellfish
Immunology 15(4): 297-310.
Chen, L.-L., C.-F. Lo, Y.-L. Chiu, C.-F. Chang and G.-H. Kou. 2000. Natural and experimental infection of
white spot syndrome virus (WSV) in benthic larvae of mud crab Scylla serrata. Diseases of Aquatic
Organisms 40(2): 157-161.
Chotigeat, W., S. Tongsupa, K. Supamataya and A. Phongdara. 2004. Effect of fucoidan on disease
resistance of black tiger shrimp. Aquaculture 233(1-4): 23-30.
Clifford, H.C. III. 1999. A review of diagnostic, biosecurity and management measures for the exclusion of
white spot virus disease from shrimp culture systems in The Americas. In T. Cabrera, D. Jory and
M. Silva, eds., Acuicultura ‘99, Vol. 1, pp.134-171. Congreso sur American de Acuicultura,
Encuentro de Genetica, Puerto la Cruz (Venezuela), 17-20 Nov. 1999.
Corbel, V., R. Zuprizal, Z. Shi, C. Huang, L. Sumartono, J-M. Arcier, J-R. Bonami. 2001. Experimental
infection of European crustaceans with white spot syndrome virus (WSSV). Journal of Fish Disease
(7): 377-382.
Corsin, F., P.C. Thakur, P.A. Padiyar, M. Madhusudhan,J.F. Turnbull, C.V. Mohan, N.V. Hao, K.L. Morgan.
Relationship between white spot syndrome virus and indicators of quality in Penaeus
monodon postlarvae in Karnataka, India. Diseases of Aquatic Organisms 54 (2): 97-104.
Dupuy, J.W., J.R. Bonami, and P. Roch. 2004. A synthetic antibacterial peptide from Mytilus
galloprovincialis reduces mortality due to white spot syndrome virus in palaemonid shrimp. Journal
of Fish Disease 27(1): 57.
Durand, S.V., K.F.J. Tang and D.V. Lightner. 2000. Frozen commodity shrimp: potential avenue for
introduction of white spot syndrome virus and yellow head virus. Journal of Aquatic Animal Health
(2): 128-135.
Flegel, T.W., D.F. Fegan and S. Sriurairatana. 1995. Environmental control of infectious shrimp diseases in
Thailand. Pp. 65-69 in M. Shariff, J.R. Arthur, and R.P. Subasinghe, eds., Diseases in Asian
Aquaculture II: Proceedings of the 2nd Symposium on diseases in Asian Aquaculture, 25-29 Oct.
, Phuket, Thailand. Fish Health Section, Asian Fisheries Society. Manila.
Flegel, T.W., S. Boonyaratpalin, and B. Withyachumnarnkul. 1997. Progress in research on yellow-head
virus and white spot virus in Thailand. In: T.W. Flegel and I.H. MacRae (Eds.), Diseases in Asian
Aquaculture III, Fish Health Section, Asian Fisheries Society, Manila. Pp.285-295.
Granda, L.A., J.A. Altbuch, Z. Xu, D.M. Meehan, A.A. Alcivar-Warren. 2001. Genetic diversity and
prevalence f white spot virus (WSV) in wild Litopenaeus vannamei from Ecuador. World
Aquaculture Society Aquaculture 2001: Book of Abstracts, p. 255.
Grillo F., Manuel, D.M. Dugger and D.E. Jory. 2000. Zero exchange shrimp production success in WSSVinfected
Panama. Global Aquaculture Advocate:Dec. 2000, pp. 55-56.
Hao, N.V., D.T. Thuy, L.T.T. Loan, T.T. Phi, L.H. Phuoc, H.H.T. Duong, F. Corsin and P. Chanratchakool.
Presence of two viral pathogens WSSV and MBV in three wild shrimp species (Penaeus
indicus, Metapenaeus ensis, and Metapenaeus lysianassa) cultured in the mangrove forest of Ca
Mau Province. Journal of Asian Fisheries Science 12(4): 309-325.
Hossain, M.S., A. Chakraborty, B. Joseph, S.K. Otta, I. Karunasagar, and I. Karunasagar. 2001. Detection of
new hosts for white spot syndrome virus of shrimp using nested polymerase chain reaction.
Aquaculture 198(1-2): 1-11.
Huang, C.-C. And Y.-L. Song. 1999. Maternal transmission of immunity to white spot syndrome associated
virus (WSSV) in shrimp (Penaeus monodon). Developmental and Comparative Immunology 23(7-
: 545-552.
Huang, C.H., L.R. Zhang, J.H. Zhang, L.C. Ziao, Q.J. Wu, D.H. Chen, and JKK Li. 2001. Purification and
characterization of White Spot Syndrome Virus (WSSV) produced in an alternate host: crayfish,
Cambarus clarkii.
Jiravanichpaisal, P., E. Bangyeekhun, K. Soederhaell and I. Soederhaell. 2001. Purification and
characterization of white spot syndrome virus (WSSV) produced in an alternate host: crayfish,
Cambarus clarkii. Diseases of Aquatic Organisms 47(2): 151-157.
Johnson, S.K. and S.L.S. Bueno. 2000. Health Management. pp. 239-257 in Freshwater Prawn Culture.
Jory, D.E. and H.M. Dixon. 1999. Shrimp white spot virus in the Western Hemisphere. Aquaculture
Magazine, May-June 1999, p. 83.
Kanchanaphum, P., C. Wongteerasupaya, N. Sitidilokratana, V. Boonsaeng, S. Panyim, A. Tassanakajon, B.
Withyachumnarnkul, and T.W. Flegel. 1998. Dis. Aquat. Org. 34(1):1-7.
Kim, C.-K., P.-K. Kim, S.-G. Sohn, D.-S. Sim, M.-A. Park, M.-S. Heo, T.-H. Lee, J.-D. Lee, H.-K. Jun, and
K.-L. Jang. 1998. Development of a polymerase chain reaction (PCR) procedure for the detection
of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimp. Journal of Fish
Disease 21(1): 11-17.
Kondo, M., T. Itami and Y. Takahashi. 1992. The phenoloxidase activity in prawn hemocytes. Gyobyo
Kenkyu 27: 185-189.
Laramore, S.E. 2002. Pathogenicity of white spot syndrome virus to the ornamental shrimp, Lysmata
wurdemanni. 4th International Symposium on Aquatic Animal Health, New Orleans, LA, USA, 2-6
Sept. 2002.
Li, G., J. Zhang, Y. Chen and F. Yang. 2003. White spot syndrome virus (WSSV) infectivity for Artemia at
different developmental stages. Diseases of Aquatic Organisms 57(3):261-264.
Lightner, D.V. 2001. The penaeid shrimp viruses TSV, IHHNV, WSSV, and YHV: current status in the
Americas, available diagnostic methods and management strategies. In, C. Lim and C.D. Webster,
Eds., Nutrition and Fish Health, pp. 79-102, Food Products Press, Binghamton, NY USA
Lightner, D.V. 2001. Research activities at the University of Arizona (UAZ). (abstract) World Aquaculture
Society 2001 Book of Abstracts:p.375.
Lightner, D.V., K. Tang-Nelson, S.V. Durand, R.M. Redman and L.L. Mohney. 2001. Qualitative and
quantitative studies on the relative virus load of tails and heads of shrimp acutely infected with
WSSV (white spot syndrome virus). Aquaculture 2001: Book of Abstracts, P. 274, World
Aquaculture Society, 21-25 Jan., Buena Vista, FL USA.
Liu, K.-F, T.-I. Chen, K.L. Horng, H.-M. Liu, M.-S. Su and I.C. Liao. 2001. Treatment of beta -1,3-glucan
increases resistance of giant tiger prawn larvae to WSSV.6th Asian Fisheries Forum, Kaohsiung
(Taiwan) 25-30 Nov 2001, 6th Asian Fisheries Forum Book of Abstracts, p. 156.
Liu, L.-C., B. Chung and C. Su. 2001. SPF culturing strategy of L. vannamei on small scale, non-integrated
farms: successful cases report in Taiwan and China. 6th Asian Fisheries Forum, Kaohsiung (Taiwan)
-30 Nov 2001, 6th Asian Fisheries Forum Book of Abstracts, p. 231.
Lo, C.-F., C.-H. Ho, S.-E. Peng, C.-H. Chen, H.-C. Hsu, Y.-L. Chiu, C.-F. Chang, K.-F. Liu, M.-S. Su, C.-H.
Wang, and G.-H. Kou. 1996. White spot syndrome baculovirus (WSBV) detected in cultured and
captured shrimp, crabs, and other arthropods. Diseases of Aquatic Organisms 27: 215-225.
Lo, C.-F., C.-H. Ho, C.-H. Chen, K.-F. Liu, Y.-L. Chiu, P.-Y. Yeh, S.-E. Peng, H.-C. Hsu, H.-C. Liu, C.-F.
Chang, M.-S. Su, C.-H. Wang and G.-H. Kou. 1997. Detection and tissue tropism of white spot
syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis
on reproductive organs. Diseases of Aquatic Organisms 30: 53-72.
Lo, C.-F., S.-E. Peng and G.-H. Kou. 1998. PCR screening for white spot syndrome virus (WSSV) in
Penaeus monodon brooders: a general effort to combat shrimp WSS. In I.C. Liao and J. Bakers
(Eds.), Aquaculture and Fisheries Management. Conference Proceedings. Taiwan Fisheries
Research Institute no. 4 pp 63-72.
Lotz, J.M. 1997. Viruses, biosecurity and specific pathogen-free stocks in shrimp aquaculture. World
Journal of Microbiology and Biotechnology 13(4): 405-413.
Madhavi, R., P. Janakiram, L. Jayasree, and P.S.N. Murthy. 2002. Occurrence of concurrent infections with
multiple viruses in Penaeus monodon from culture ponds of north coastal Andhra Pradesh. Current
Science 82(11): 1397-1400.
Maeda, M., J. Kasornchandra, T. Itami, N. Suzuki, O. Hennig, M. Kondo, J.D. Albaladejo, and Y. Takahashi.
Effect of various treatments of white spot syndrome virus (WSSV) from Penaeus japonicus
(Japan) and P. monodon (Thailand) Fish Pathology 33(4): 381-387.
Maeda, M., T. Itami, E. Mizuki, R. Tanaka, Y. Yoshizu, K. Doi, C. Yasunaga-Aoki, Y. Takahashi, and T.
Kawarabata. 2000. Red swamp crawfish (Procambarus clarkii): An alternative experimental host in
the study of white spot syndrome virus. Acta Virologica. 44(6): 371-374.
Magbanua, F.O., K.T. Natividad, V.P. Migo, C.G. Alfafara, F.O. de la Pena, R.O. Miranda, J.D. Albaladejo,
E.C.B. Nadala Jr., P.C. Loh and L.Mahilum-Tapay. 2000. White spot syndrome virus (WSSV) in
cultured Penaeus monodon in the Philippines. Diseases of Aquatic Organisms 42(1): 77-82.
Manivannan, S., S.K. Otta, I. Karunasagar and I. Karunasagar. 2002. Multiple viral infection in Penaeus
monodon shrimp postlarvae in an Indian hatchery. Diseases of Aquatic Organisms 48(3): 233-236.
Mazid, M.A. and A.N.H. Banu. 2002. An overview of the social and economic impact and management of
fish and shrimp disease in Bangladesh, with an emphasis on small-scale aquaculture. Primary aquatic
animal health care in rural, small-scale aquaculture development. Technical proceedings of the Asia
Regional scooping workshop, Dhaka, Bangladesh, 27-30 September 1999. FAO Fish. Tech. Pap.
No. 406, pp. 21-25.
Momoyama, K., M. Hiraoka, H. Nakano and M. Sameshima. 1998. Cryopreservation of penaeid rod-shaped
DNA virus (PRDV) and its survival in sea water at different temperatures. Fish Pathology 33(2): 95-
Nair, M.R. 2000. History and present status of white spot baculovirus (WSBV) and other shrimp diseases in
India. Journal of the World Aquaculture Society 31(2): 10-13.
Namikoshi, A., J.L. Wu, T. Yamashita, T. Nishizawa, T. Nishioka, M. Arimoto and K. Muroga. Vaccination
trials with Penaeus japonicus to induce resistance to white spot syndrome virus. Aquaculture 229(1-
: 25-35.
Nunan, L.M., B.T. Poulos and D.V. Lightner. 1998. The detection of white spot syndrome virus (WSSV)
and yellow head virus (YHV) in imported commodity shrimp. Aquaculture 160(1-2): 19-30.
Nunan, L.M., B.B. Poulos and D.V. Lightner. 1998. Reverse transcription polymerase chain reaction (RTPCR)
used for the detection of Taura Syndrome Virus in experimentally infected shrimp. Diseases
of Aquatic Organisms 34(2): 87-91.
Nunan, L.M., S.M. Arce, R.J. Staha and D.V. Lightner. 2001. Prevalence of infectious hypodermal and
hematopoietic necrosis virus (IHHNV) and white spot syndrome virus (WSSV) in Litopenaeus
vannamei in the Pacific Ocean off the coast of Panama. Journal of the World Aquaculture Society
(3):330-334.
Otta, S.K.,I Karunasagar, and I. Karunasagar. 2003. Detection of monodon baculovirus and white spot
syndrome virus in apparently healthy Penaeus monodon postlarvae from India by polymerase chain
reaction. Aquaculture 220(1-4):59-67.
Peng, S.-E., C.-F. Lo, S.-C. Lin, L.-L. Chen, Y.-S. Chang, K.-F. Liu, M.-S. Su and G.-H. Kou. 2001.
Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds.
Diseases of Aquatic Organisms 46:165-172.
Pongmaneerat, J., J. Kasornchandra, S. Boonyaratpalin and M. Boonyaratpalin. 2001. Effect of dietary
shrimp head meal contaminated with white spot syndrome virus (WSSV) on detection of WSSV in
black tiger shrimp (Penaeus monodon Fabricius). Aquaculture Research 32(1): 383-387.
Pramod Kiran, R.B., K.V. Rajendran, S.J. Jung, and M.J. Oh. 2002. Experimental susceptibility of different
life-stages of the giant freshwater prawn, Macrobrachium rosenbergii (de Man), to white spot
syndrome virus (WSSV). Journal of Fish Disease 25(4): 201-208.
Prior, S. and C.L. Browdy. 2000. Postmortem persistence of white spot and taura syndrome viruses in water
and tissue. Book of Abstracts, p. 402. World Aquaculture Society, U.S. Chapter annual conference,
-5 Feb., New Orleans, U.S.
Qingyin, W., and K. Jie. 2002. Genetic advances with fleshy shrimp in China. Global Aquaculture
Advocate, pp. 51-52, Dec. 2002.
Rajendran, K.V., K.K. Vijayan, T.C. Santiago and R.M. Krol. 1999. Experimental host range and
histopathology of white spot syndrome virus (WSSV) infection in shrimp, prawns, crabs and lobsters
from India. Journal of Fish Disease 22(3):183-191.
Rodriguez, J, B. Bayot, Y. Amano, F. Panchana, I. de Blas, V. Alday and J. Calderon. 2003. White spot
syndrome virus infection in cultured Penaeus vannamei (Boone) I Ecuador with emphasis on
histopathology and ultrastructure. Journal of Fish Disease 26(8):439-450.
Sahul Hameed, A.S. 2001. White spot syndrome virus (WSSV) in two species of freshwater crabs
(Paratelphusa hydrodomous and P. pulvinata). Aquaculture 201(3-4):179-186.
Sahul Hameed, A.S., M.X. Charles and M. Anilkumar. 2000. Tolerance of Macrobrachium rosenbergii to
white spot syndrome virus. Aquaculture 183(3-4):207-213.
Sahul Hameed, A.S., B.L.M. Murthi, M. Rasheed, S. Sathish, K. Yoganandhan, V. Murugan, and K.
Jayaraman. 2002. An investigation of Artemia as a possible vector for white spot syndrome virus
(WSSV) transmission to Penaeus indicus. Aquaculture 204(1-2):1-10.
Shi, Z., C. Huang, J. Zhang, D. Chen and J.R. Bonami. 2000. White spot syndrome virus (WSSV)
experimental infection of the freshwater crayfish, Cherax quadricarinatus. Journal of Fish Disease
(4): 285-288.
Song, Y-.L., J.-J. Liu, L.-C. Chan and H.-H. Sung. 1997. Glucan-induced disease resistance in tiger shrimp
(Penaeus monodon). In: R. Gudding, A. Lillehaug, P.J. Midtlyng, and F.Brown, eds., Fish
Vaccinology. Developments in biological standardization. Basel:Karger, 90: 335-343.
Soto, M.A., V.R. Shervette and J.M. Lotz. 2000. Transmission of white spot syndrome virus (WSSV) to
Litopenaeus vannamei from infected cephalothorax, abdomen, or whole shrimp cadaver. Diseases of
Aquatic Organisms 45(2): 81-87.
Soto, M.A. and J.M. Lotz. 2001. Epidemiological parameters of white spot syndrome virus infections in
Litopenaeus vannamei and L. setiferus. Journal of Invertebrate Pathology 78(1): 9-15.
Soto, M.A., V.R. Shevetter, and J.M. Lotz. 2001. Susceptibility of Litopenaeus vannamei, Farfantepenaeus
duorarum, to White Spot Syndrome Virus (WSV) and infection of Menippe adina with WSSV.
Proceedings of the Fifty-Third Annual Gulf and Caribbean Fisheries Institute. Proceedings of the
Gulf & Caribbean Fisheries Institute 53: 38-45.
Sung, H.-H., G.-H. Kou and Y.-L. Song. 1996. Vibriosis resistance induced by glucan treatment in tiger
shrimp (Penaeus monodon). Fish Pathology 29: 11-17.
Sung, H.-H., H.-J. Chang, C.-H. Her, J.-C. Chang and Y.-L. Song. 1998. Phenoloxidase activity of
hemocytes derived from Penaeus monodon and Macrobrachium rosenbergii. Journal of Invertebrate
Pathologoy 71: 26-33.
Supamattaya, K., R.W. Hoffmann, S. Boonyaratpalin and P. Kanchanaphum. 1998. Experimental
transmission of white spot syndrome virus (WSSV) from black tiger shrimp Penaeus monodon to the
sand crab Portunus pelagicus, mud crab Scylla serrata and krill Acetes sp. Diseases of Aquatic
Organisms 32(2): 79-85.
Takahashi, Y., M. Kondo, T. Itami, T. Honda, H. Inagawa, T. Nishizawa, G. Soma and Y. Yokomizo. 2000.
Enhancement of disease resistance against penaeid acute viraemia and induction of virus-inactivating
activity in haemolymph of kuruma shrim, Penaeus japonicus, by oral administration of Pantoea
agglomerans lipopolysaccharide (LPS). Fish & Shellfish Immunology 10(6): 555-558.
Thakur, P.C., F. Corsin, J.F. Turnbull, K.M. Shankar, N.V. Hao, P.A. Padiyar, M. Madhusudhan, K.L.
Morgan, and C.V. Mohan. 2002. Estimation of prevalence of white spot syndrome virus (WSSV)
by polymerase chain reaction in Penaeus monodon postlarvae at time of stocking in shrimp farms of
Karnataka, India: a population-based study. Diseases of Aquatic Organisms 49(3): 235-243.
Tsai, M.-F., G.-H. Kou, H.-C. Liu, K.-F. Liu, C.-F. Chang, S.-E. Peng, H.-C. Hsu, C.-H. Wang and C.-F. Lo.
Long-term presence of white spot syndrome virus (WSSV) in a cultivated shrimp population
without disease outbreaks. Diseases of Aquatic Organisms 38(2): 107-114.
Umesha, R.K., A. Uma, S.K. Otta, I. Karunasagar, and I. Karunasagar. 2003. Detection by PCR of
hepatopancreatic parvovirus (HPV) and other viruses in hatchery-reared Penaeus monodon
postlarvae. Diseases of Aquatic Organisms 57(1-2): 141-146.
Vanpatten, Kristie A., L.M. Nunan, B.T. Poulos and D.V. Lightner. 2004. Birds as a mechanism of viral
transmission of WSSV, TSV, YHV and IHHNV: infectivity studies in Litopenaeus vannamei.
(Abstract) World Aquaculture Society Aquaculture 2004, Honolulu, HI, USA. Book of Abstracts:
p.608.
Van Hulten, M.C., J. Witteeveldt, M. Snippe and J.M. Vlak. White spot syndrome virus envelope protein
VP28 is involved in the systemic infection of shrimp. Virology 285(2): 228-233.
Vaseeharan, B., R. Jayakumar and P. Ramasamy. 2003. PCR-based detection of white spot syndrome virus
in cultured and captured crustaceans in India. Letters in Applied Microbiology 37(6): 443-447.
Venegas, C.A., L. Nonaka, K. Mushiake, T. Nishizawa, K. Muroga. 2000. Quasi-immune response of
Penaeus japonicus to penaeid rod-shaped DNA virus (PRDV). Diseases of Aquatic Organisms
(2): 83-89.
Vidal, O.M., C.B. Granja, F. Aranguren, J.A. Brock and M. Salazar. 2001. A profound effect of
hyperthermia on survival of Litopenaeus vannamei juveniles infected with white spot syndrome
virus. Journal of the World Aquaculture Society 32(4): 364-372.
Withyachumnarnkul, B. 1999. Results from black tiger shrimp Penaeus monodon culture ponds stocked with
postlarvae PCR-positive or -negative for white-spot virus (WSSV). Diseases of Aquatic Organisms
: 21-27.
Withyachumnarnkul, B., V. Boonsaeng, R. Chomsoong, T.W. Flegel, S. Muangsin, and G.L. Nash. 2003.
Seasonal variation in white spot syndrome virus-positive samples in broodstock and post-larvae of
Penaeus monodon in Thailand. Diseases of Aquatic Organisms 53(2): 167-171.
Witteveldt, J., C.C. Cifuentes, J.M. Vlak and M.C.W. Van Hulten. 2004. Protection of Penaeus monodon
against white spot syndrome virus by oral vaccination. Journal of Virology 78(4): 2057-2061.
Wongteerasupaya, C., W. Tongchuea, V. Boonsaeng, S. Panyim, A. Tassanakajon, B. Withyachumnarnkul
and T.W. Flegel. 1997. Detection of yellow-head virus (YHV) of Penaeus monodon by RT-PCR
amplification. Diseases of Aquatic Organisms 31(3): 181-186.
Wu, J.L., A. Namikoshi, T. Nishizawa, K. Mushiake, K. Teruya and K. Muroga. 2001. Effects of shrimp
density on transmission of penaeid acute viremia in Penaeus japonicus by cannibalism and the
waterborne route. Diseases of Aquatic Organisms 47(2): 129-135.
Wu, J.L., T. Nishioka, K. Mori, T. Nishizawa and K. Muroga. 2002. A time-course study on the resistance of
Penaeus japonicus induced by artificial infection with white spot syndrome virus. Fish & Shellfish
Immunology 13(5): 391-403.
Yan, D.-C., Dong, S.L., Huang, J, X.-M. Yu, M.-Y. Feng and X-Y Liu. 2004. White spot syndrome virus
(WSSV) detected by PCR in rotifers and rotifer resting eggs from shrimp pond sediments. Diseases
of Aquatic Organisms 59(1): 69-73.
Yi, G., J. Qian, Z. Wang and Y. Qi. 2003. A phage-displayed peptide can inhibit infection by white spot
syndrome virus of shrimp. Journal of General Virology 83(9): 2545-2553.
Yu, Z., C. Li, and Y. Guan. 2003. Effect of salinity on the immune responses and outbreak of white spot
syndrome in the shrimp Marsupenaeus japonicus. Ophelia 57(2): 99-106.
Zhang, X., C. Huang, and Q. Qin. 2004. Antiviral properties of hemocyanin isolated from shrimp Penaeus
monodon. Antiviral Research 61(2): 93-99.
Zhu, J. and C. Lu. 2001. Characterization of shrimp white spot syndrome virus (WSSV) infection in
Procambarus clarkii. Journal of Fisheries of China/Shuichan Xuebao (Shanghai): 25(1): 47-51.