Implementación de un Bioensayo in vitro con Células Disociadas de Hepatopáncreas de Palaemon serratus y Procambarus bouvieri para Detectar la Secreción de la a-Amilasa.
Resumen
En los crustáceos la digestión comienza en la cavidad cardíaca del estómago y se continúa
en los túbulos del hepatopáncreas (Vonk, 1960). Es a nivel de esta glándula que la digestión
se hace más activa (Van Wormhoudt, 1980), asegurada por enzimas secretadas por células
especializadas (Barker y Gibson, 1977). Cinco tipos celulares han sido descritos: las células E
(embryonic cells), las células R (resorptive cells), las células F (fibrilar cells), las células B
(blister-like cells) y las células M (midget cells) (Al-Mohanna et al., 1985a,b; Al-Mohanna y
Nott, 1987a,b; Vogt, 1994). El porcentage de cada tipo de células varia en función del estadio
de muda, las células F, responsables de la síntesis y de la secreción de las enzimas
digestivas se encuentran en mayor concentración en la intermuda (estadio C) (Toullec et al.,
1992).
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