CARACTERIZACION DE LAS TRIPSINAS Y AMILASAS DE PENAEUS VANNAMEI (CRUSTACEA DECAPODA): ADAPTACIIN A LA COMPOSICION DEL REGIMEN ALIMENTICIO
Resumen
Las tripsinas, entre las proteasas, y las amilasas, entre las carbohidrasas, son las enzimas más activas en Penaeus vannamei, un camarón de interés comercial, especialmente en Latinoamérica. Estas enzimas y sus cDNAS fueron caracterizados. En lo que respecta a las tripsinas, se reconocieron cinco isoformas y se analizaron sus cDNAs correspondientes, los cuales codifican una preproenzima de 225 aminoácidos conteniendo un supuesto precursor peptídico de 14 residuos y una secuencia señal altamente hidrofobica de 14 aminoácidos. Dentro de la secuencia del zimogeno, se detectaron algunos sitios autocatalíticos (Int. J. Biochem. Cell Biol. 1996, 28, 551-563). Con respecto a las amilasas, dos isoformas fueron purificadas y caracterizadas; se hizo una selección en una biblioteca de cDNAs hepatopáncreaticos fue cribada, y se aisló y determinó la secuencia completa de los dos cDNAs correspondientes. Se caracterizó una preenzima de 511 residuos conteniendo una señal hidrofóbica de 16 aminoácidos (J. Mol. Evol., 1996, 42, 543-551).Descargas
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