Las Algas Marinas de los Géneros Byothamnion y Halimeda como Fuentes de Antioxidantes Naturales

Autores/as

  • Alexis de Jesus Vidal Novoa Universidad de La Habana
  • Jorge Mancini-Filho Universidad de La Habana
  • Daylin Diaz Gutierrez Universidad de La Habana
  • Adyary Fallarero Linares Universidad de La Habana

Palabras clave:

Algas marinas, antioxidantes, Halimeda, Bryothamnion, polifenoles

Resumen

El objetivo de este trabajo es analizar y comparar las propiedades antioxidantes mediante ensayos in vitro de extractos acuosos de las algas de los géneros Bryothamnion y Halimeda así como esclarecer sus posibles mecanismos de acción.
Se obtuvieron los siguientes resultados: Bryothamnion: DPPH; CI50=1,15 ± 0,06, capacidad reductora; 128 mg/mL, DO=2,798, inhibición de la peroxidación lipídica; CI50=5,09± 0,25, inhibición de la hemólisis con 5 mg/mL; 100 %, ensayo del β-caroteno-ácido linoléico; 1mg de extracto 15% de inhibición y Capacidad antioxidante mediante el ensayo xantina/xantina oxidasa como generador de radicales O2; no presenta actividad. Halimeda: DPPH; CI50=12,34 ± 0,30 mg/mL, capacidad reductora; DO=0,800, inhibición de la peroxidación lipídica; CI50=1,25± 0,31 mg/mL, inhibición de la hemólisis; 24%, ensayo del β-caroteno-ácido linoléico; 1mg de extracto 68% de inhibición y Capacidad antioxidante mediante el ensayo xantina/xantina oxidasa como generador de radicales O2; muy efectivo. Las algas de los dos géneros resultaron muy efectivas en los estudios de cultivos de células así como en el modelo de estrés oxidativo inducido por CCl4 en ratas Wistar.
Al comparar los resultados de las propiedades antioxidantes entre las algas se comprobó que Bryothamnion resultó mucho más eficiente en algunas metodologías mientras Halimeda resulta más eficiente en otros ensayos.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Abdel-Wahhab MA, Ahmed HH, Hagazi MM. Prevention of aflatoxin B1-initiated hepatotoxicity in rat by marine algae extracts. J Appl Toxicol 2006; 26(3):229–38.

Anantharaman P, Karthikaidevi G, Manivannan K, Thirumaran G, Balasubramanian T. Mineral composition of marine macroalgae from mandapam coastal regions; southeast coast of India Rec Res Sci Tech 2010; 2(10): 66-71.

Batista-Gonzalez AE, de Oliveira e Silva AM, Vidal-Novoa A, Pinto JR, Portari Mancini DA, Mancini-Filho J. Analysis of antioxidant properties of hydrophilic fractions from seaweed Halimeda monileL. and its function in vivo. J Food Biochem. 2012; 36: 189–97.

Benites Vílchez J, Díaz García R, López Vivar J, Gajardo Solari S, Kusch Fuschlocher F, Rojas Arredondo M. Actividad antioxidante y antibacteriana de seis cáscaras de frutos del oasis de Pica. Biofarbo. 2011; 19(1):1-7.

Boonchum W, Peerapornpisal Y, Kanjanapoth D, Pekkoh J, Pumas C, Jamjai U, et al. Antioxidant activity fo some seaweed from the Gulf of Tailand. Int J Agric Biol. 2011; 13(1):95-9.

Bupesh G, Amutha C., Vasanth, S., Manoharan, N., Senthil Raja, R., Krishnamoorthy, R., Subramanian, P., Hepatoprotective Efficacy of Hypnea muciformis Ethanolic Extract on CCl4 Induced Toxicity in Rats. Braz Arch Biol Technol 2012; 55 (6): 857-863.

Chakraborty K, Praveen NK, Vijayan KK, Rao GS. Evaluation of phenolic contents and antioxidant activities of brown seaweeds belonging to Turbinaria spp. (Phaeophyta, Sargassaceae) collected from Gulf of Mannar. Asian Pac J Trop Biomed. 2013; 3(1):8-16.

Costa-Mugica A, Batista-Gonzalez AE, Mondejar D, Soto-López Y, Brito-Navarro V, Vázquez AM, et al. Inhibition of LDL-oxidation and antioxidant properties related to polyphenol content of hydrophilic fractions from seaweed Halimeda Incrassata (Ellis) Lamouroux. Braz J Pharm Sci. 2012; 48 (1): 31-7.

Dutra Rocha F, Crespo Pereira R, Coelho Kaplan MA, Laneuville Teixeira V. Produtos naturais de algas marinhas e sue potencial antioxidante. Braz J Pharmacogn. 2007; 17(4): 631-39.

Fallarero A, Peltoketo A, Loikkanen JJ, Tammela P, Vidal A, Vuorela P. Effects of aqueous extracts of Bryothamnion triquetrum on chemical hypoxia and aglycemia-induced damage in GT1-7 mouse hypothalamic immortalized cells. Phytomedicine 2006; 13(4):240-45.

Fallarero A, Peltoketo A, Loikkanen JJ, Tammela P, Vidal A, Vuorela P. Effects of aqueous extracts of Bryothamnion triquetrum on chemical hypoxia and aglycemia-induced damage in GT1-7 mouse hypothalamic immortalized cells. Phytomedicine. 2006; 13(4):240-5.

Fukumoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 2000; 48: 3597–3604.

Vidal, A., et al. 2015. Las Algas Marinas de los Géneros Byothamnion y Halimeda como Fuentes de Antioxidantes Naturales. En: Cruz-Suárez, L.E.,

Ricque-Marie, D., Tapia-Salazar, M., Nieto-López, M.G., Villarreal-Cavazos, D. A., Gamboa-Delgado, J., Rivas Vega, M. y Miranda Baeza, A. (Eds),

Nutrición Acuícola: Investigación y Desarrollo, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México,

ISBN 978-607-27-0593-7,pp. 183-219.

Gomez –Gutierrez CM, Guerra-Rivas G, Soria-Mercado IE, Ayala-Sánchez NE. Marine edible algae as disease preventers. Adv Food Nutr Res. 2011; 64: 29-39.

Heck S, Lezoualc’h F, Engert S, Behl C. Insulin-like growth factor-1-mediated neuroprotection against oxidative stress is associated with activation of nuclear factor ĸB. J Biol Chem 1999; 274: 9828–9835

Josephine A, Nithya K, Amudha G, Veena CK, Preetha SP, Varalakshmi P. Role of sulphated polysaccharides from Sargassum wightii in Cyclosporine A-induced oxidative liver injury in rats. BMC Pharmacology 2008; 8:4.

Kang, KA, Lee KH, Chae S, Koh YS, Yoo B-S, Kim JH, Ham YM, Baik JS, Lee NH, Hyun JW. Triphlorethol-A from Ecklonia cava protects V79-4 ling fibroblast against hydrogen peroxide induced cell damage. Free Radic. Res. 2005; 39(8), 883–892.

Kaur G, AlamS, Jabbar Z, Javed K, Athar M. Evaluation of antioxidant activity of Cassia siamea flowers. J Ethnopharmacol. 2006; 108:340-8.

Kim H-Y, Kim J-H, Lee S-A, Chang H-E, Park M-H, Hwang S-J, et al. Saengshik, a formulated health food, prevents liver damage in CCl4-induced mice and increases antioxidant activity in elderly women. J Med Food 2008; 11(2):323–330.

Kim H-Y, Kim J-H, Lee S-A, Chang H-E, Park M-H, Hwang S-J, et al. Saengshik, a formulated health food, prevents liver damage in CCl4-induced mice and increases antioxidant activity in elderly women. J Med Food 2008; 11(2):323–330.

Kuda T, Ikemori T. Minerals, polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beach-casts in the Noto Peninsula, Ishikawa, Japan. Food Chem. 2009; 112: 575-81.

Lim CS, Jin DQ, Sung JY, Lee JH, Choi HG, Há I, et al. Antioxidant and anti-inflamatory activities of the methanolic extract of Neorhodomela aculeate in Hippocampal and Microglial cells. Biol Pharm Bull. 2006; 29(6): 1212–6.

Lim SN, Cheung PCK, Ooi VEC, Ang PO. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J. Agric Food Chem. 2002; 50:3862-6.

Linares AF, Loikkanen J, Jorge MF, Soria RB, Novoa AV. Antioxidant and neuroprotective activity of the extract from the seaweed, Halimeda incrassata (Ellis) Lamouroux, against in vitro and in vivo toxicity induced by methyl-mercury. Vet Hum Toxicol 2004; 46(1): 1-5.

Loikkanen JJ, Naarala J, Savolainen KM. Modification of glutamate-induced oxidative stress by lead: the role of extracellular calcium. Free Radic Biol Med 1998; 24: 377–384.

Lu SC. Regulation of hepatic glutathione synthesis: current concepts and controversies. FASEB J 1999, 13: 1169-1183.

Mac Artain P, Gill CIR, Brooks M, Campbell R, Rowland IR. Nutritional value of edible seaweeds. Nutr Rev. 2007; 65(12): 535-43.

Vidal, A., et al. 2015. Las Algas Marinas de los Géneros Byothamnion y Halimeda como Fuentes de Antioxidantes Naturales. En: Cruz-Suárez, L.E.,

Ricque-Marie, D., Tapia-Salazar, M., Nieto-López, M.G., Villarreal-Cavazos, D. A., Gamboa-Delgado, J., Rivas Vega, M. y Miranda Baeza, A. (Eds),

Nutrición Acuícola: Investigación y Desarrollo, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México,

ISBN 978-607-27-0593-7,pp. 183-219.

Matsukawa R, Dubinsky Z, Kishimoto E, Masaki K, Masuda Y, Takeuchi T, et al. A comparison of screening methods for antioxidant activity in seaweeds. J Appl Phycol. 1997; 9: 29-35.

McCord JM, Fridovich I. Superoxide dismutase, an enzyme function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244:6049–55.

Mller, HEA. A simplified method for the evaluation of antioxidants. J Am Oil Chem Soc 1971; 48: 91.

Moon J-K, Shibamoto T. Antioxidant assays for plant and food components. J Agric Food Chem. 2009; 57(2): 1655-66.

Nakai M, Kageyama N, Nakahara K, Miki W. Phlorotannins as radical scavengers from the extract of Sargassum ringgoldianum. Mar Biotechnol. 2006; 8: 409-14.

Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K. Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology 2003; 62:353–6.

Pannangpetch P, Laupattarakasem P, Kukongviriyapan V, Kukongviriyapan U, Kongyingyoes B, Aromdee C. Antioxidant activity and protective effect against oxidative hemolysis of Clinacanthus nutans (Burm.f) Lindau. Songklanakarin. J Sci Technol. 2007; 29 (Suppl. 1): 1-9.

Proksch P, Edrada-Ebel RA, Ebel R. Drugs from the sea- Opportunities and obstacles. Mar Drugs. 2003; 1: 5-17.

Punitha SC, Rajasekaran M. antioxidant mediated defense role of Wedelia calendulacea herbal extract against CCl4 induced toxic hepatitis. J Appl Pharmac Sci 2011; 01(09):111–5.

Puppo A. Effect of flavonoids on hydroxyl radical formation by Fenton-type reactions; influence of the iron chelator. Phytochemistry. 1992; 31: 85–8.

Raghavendran HR, Sathivel A, Devaki T. Effect of Sargassum polycystum (Phaeophyceae)-sulphated polysaccharide extract against acetaminophen-induced hyperlipidemia during toxic hepatitis in experimental rats. Mol Cell Biochem 2005; 276: 89–96.

Raghavendran HR, Sathivel A, Devaki T. Protective effect of Sargassum polycystum (brown alga) against acetaminophen-induced lipid peroxidation in rats. Phytother. Res 2005; 19 (2): 113-115. Recknagel RO. 1967. Carbon tetrachloride hepatotoxicity. Pharmacol Rev 1967; 19(2):145-208. Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res. 1995; 22(4):375-83.

Rivero F, Fallararo A, Castañeda O, Dajas F, Manta E, Areces A, et al. Antioxidant activity in vivo and in vitro of Halimeda incrassata aqueous extracts. Cienc Tecnol Alimentos (Campinas) 2003; 23: 256 –63.

Sampth-Wiley P, Neefus CD, Jahnke LS. Seasonal effects of sun exposure and emersion on intertidal seaweed physiology: fluctuations in antioxidant contents, photosynthetic pigments and photosynthetic efficiency in the red alga Phorphyra umbilicalisKutzing (Rodophyta, Bangiales). J Exp Mar Biol Ecol. 2008; 36: 83-91.

Vidal, A., et al. 2015. Las Algas Marinas de los Géneros Byothamnion y Halimeda como Fuentes de Antioxidantes Naturales. En: Cruz-Suárez, L.E.,

Ricque-Marie, D., Tapia-Salazar, M., Nieto-López, M.G., Villarreal-Cavazos, D. A., Gamboa-Delgado, J., Rivas Vega, M. y Miranda Baeza, A. (Eds),

Nutrición Acuícola: Investigación y Desarrollo, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, México,

ISBN 978-607-27-0593-7,pp. 183-219.

Schroeter H, Williams RJ, Matin R, Iversen L, Rice-Evans CA. Phenolic antioxidants attenuate neuronal cell death following uptake of oxidized low-density lipoprotein. Free Rad Biol Med 2000; 29 (12): 1222–1233

Silva AM de O, Vidal-Novoa A, Batista-González AE, Pinto JR, Portari Mancini DA, Reina-Urquijo W, et al. Antioxidant activity and hepatoprotectivie properties of polyphenols in vitro and in vivo from seaweeds Halimeda opuntia (Linnaeus) Lamouroux. Redox report. 2012; 17 (2): 47-53.

Stevenson DE, Hurst RD. Polyphenolic phytochemicals–just antioxidants or much more? Cell Mol Life Sci 2007; 64:2900–16.

Vauzour D, Rodriguez-Mateos A, Corona G, Oruna-Concha MJ, Spencer JPE. Polyphenols and human health: prevention of disease and mechanisms of action. Nutrients. 2010; 2: 1106-31.

Vidal A, Fallarero A, de Andrade-Wartha ERS, de Oliveira e Silva AM, de Lima A, Pavan R, et al. Composición química y actividad antioxidante del alga marina roja Bryothamnion triquetrum (S.G.Gmelin) Howe. Braz J Pharm Sci. 2006; 43(4): 589-600.

Vidal A, Motidome M, Mancini J, Fallarero A, Tanae MM, Brandao LM, et al. Actividad antioxidante y ácidos fenólicos del alga marina Bryothamnion triquetrum (SG Gmelim) Howe. Braz J Pharm Sci. 2001; 37(3), 373–82.

Vidal A, Silva de Andrade-Wartha ER, de Oliveira e Silva AM, Pavan R, Lima A, Fallarero A, et al. Actividad antioxidante y polifenoles de algas marinas verdes Halimeda opuntia y Halimeda monile. Ars Pharm. 2009; 50 (1): 24-31.

Yeh C-T, Yen G-C. Induction of hepatic antioxidant enzymes by phenolic acids in rats is accompanied by increased levels of multidrug resistance – associated protein 3 mRNA expression. J Nutr 2006; 136:11–5.

Yoon Y, Kim K, Hong S, Kang B, Lee M, Cho. Protective effects of Orostachys japonicus A. Berger (Crassulaceae) on H2O2-induced apoptosis in GT1-1 mouse hypothalamic neuronal cell line. J Ethnopharmacol 2000; 69: 73–78.

Yoshie Y, Wang W, Hsieh YP, Suzuki T. Compositional difference of phenolic compounds between two seaweeds, Halimeda spp. J Tokyo Univ Fish.. 2002; 88: 21-4.

Zhang Q, Li N, Liu X, Zhao Z, Li Z, Xu Z. The structure of a sulphated galactan from Porphyra haitanensis and its in vivo antioxidant activity. Carbohydr Res 2004; 339:105–11.

Zhu H, He M, Bannenberg GL, Moldéus P, Shertzer HG. Effects of glutathione and pH on the oxidation of biomarkers of cellular oxidative stress. Arch Toxicol 1996; 70: 628–634.

Zubia M, Fabre MS, Kerjean V, Deslandes E. Antioxidant and cytotoxic activities of some red algae (Rhodophyta) from Brittany coasts (France) Bot Mar. 2009; 52: 268-77.

Zubia M, Robledo D, Freile-Pelegrin Y. Antioxidant activities in tropical marine macroalgae from the Yucatan Peninsula, Mexico. J Appl

Descargas

Publicado

2015-11-30

Cómo citar

Vidal Novoa, A. de J., Mancini-Filho, J., Diaz Gutierrez, D., & Fallarero Linares, A. (2015). Las Algas Marinas de los Géneros Byothamnion y Halimeda como Fuentes de Antioxidantes Naturales. Avances En Nutrición Acuicola. Recuperado a partir de https://nutricionacuicola.uanl.mx/index.php/acu/article/view/41